Metabolite Profiling Foamy Substance of Cucumis sativus L. white local cultivar studied by HR-LCMS

Authors

  • K. Latha Associate Professor Biotechnology, Maharanis Science College, for women Mysore–570005 Karnataka, India. Author

DOI:

https://doi.org/10.47392/IRJAEM.2024.0014

Keywords:

Resolution Liquid Chromatography, High- Mass spectrometry, Gitoxigenin, L. Cucurbita in Cardiac glycosides, Cucumis sativus

Abstract

Cucumis sativus L. belong to Cucurbitaceous. The Phytochemical profiling of white foamy substance was performed using HR-LCMS, details of projecting compounds with MS spectra, peak list, and compound structure were studied. The prominent constituent analysis (PCA) of the investigative data displayed the existence of Triterpenes: Gitoxigenin (7.76ppm), Strophanthidin(13.79ppm), Cucurbita in-C (-0.8ppm), Cucurbita in-E(5.53ppm), and Cardiac glycosides: and Digitoxigenin(-2.13ppm) in 10µl of the loaded sample.100 other composites were identified by accurate mass of Q-TOF/MS and confirmed by database compounds (IRM calibration) which comprises, abscisic acid amino acids, fatty acids pyridylactic acid etc. Strophanthidin in the premier attention in the white foamy extract is a noteworthy finding where it has interaction with various proteins in biochemical reactions. This study reports the customary implication of removing white foamy substances before slicing for ingestion. It is used as both vegetables as well as fruit in Indian traditional medicine. It is a source of bioactive compounds.

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Published

2024-03-16