U.V Spectroscopic Method for the determination of Dissolution and Assay of Aceclofenac SR60%W/W Pellets

Abstract

Aceclofenac (C16H13Cl2NO4), chemically known as [(2-{2, 6-dichlorophenyl) amino} phenyl aceto oxyacetic acid], is a crystalline powder and its molecular weight is 354.19. It is practically insoluble in water with good permeability. In-house formulation was performed related dissolution test method was proposed for the determination of Aceclofenac SR60%w/w pellets. Apparatus USP-I (basket), Media: pH-6.8 phosphate Buffer, volume 900 ml per flask Temperature: 37°C±0.5°C velocity: 50RPM Time interval: 1st, 4th, 8th hours. The determination was accomplished by U.V Photo Spectrometer at 275nm. In house limit for dissolution of Aceclofenac in 1st hour 35-45, 4th hour 50-75, 8th hour not less than 80% in phosphate buffer pH 6.8. The proposed   method was validated in terms of the accuracy, repeatability, linearity. Linearity was obeyed in the range of 2.5-5mg of Aceclofenac and repeatability (%RSD :< 0.2) was satisfactory. Dissolution and assay studies of Aceclofenac Pellets were calculated by the proposed method and obtained results were good.