Anti-Inflammatory and Anti-Cancer Activity of Shoot Tip Exudate of Gardenia Resinifera Roth

Abstract

Gardenia resinifera Roth (Rubiaceae), different parts of this plant like leaf, fruit and bark are detailed to be a part of traditional medicine system as a cure for extensive ailments. In plant-based isolation and standardisation of bioactive compounds, plenty of plant samples were required. Shoot tip exudate can be collected without harming plant but require time and patience. The pungent smell and taste of the shoot tip is an indication for volatile phytochemicals in the exudate. Hence this study rivets on the bioactive potential of Gardenia resinifera shoot tip exudate especially invitro anti-inflammatory and anticancer studies. The result of the study shows the implicit possibility of shoot tip exudate for the identification of bioactive compounds with anti-inflammatory and anticancer properties. The shoot tip exudate indicated its conquest efficiency of curbing COX and LOX enzyme in inflammation development. MTT assay uncovered the anticancer property of shoot tip exudate which were comparable with standard. Gas Chromatography with Mass Spectral analysis revealed the presence of five compounds which were already reported to have different bioactive properties. This study clearly explains the hidden possibility of bioactive efficacy of Gardenia resinifera shoot tip exudate.